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pslik tgfbr3 ha neo (Addgene inc)

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Addgene inc pslik tgfbr3 ha neo

Dependence of prevalent coreceptor trends on additional ligand characteristics and biochemical validation of engineered ENG -/- Tgfbr3-HA MCF10A cells. Related to . (A) Low-affinity ligands with high k rD for A (≡ R II ) favor the +,– trend and disfavor the – trend. (B) Ligands with higher affinity for B (≡ R I ) than for C (≡ coreceptor) favor the +,– trend. (C) Immunoblot of parental MCF10A-5E cells (+/+) and FACS sorted MCF10A-5E ENG -/- cells (–/–) for ENG with tubulin and p38 used as loading controls. (D) Immunoblot of MCF10A-5E ENG -/- stably transduced with <t>pSLIK-Tgfbr3-HA,</t> treated with or without 1 µg/ml doxycycline (DOX) for 24 hours, and probed for Tgfbr3 and HA tag with tubulin and ERK1/2 used as loading controls. (E) Representative biplots of Tgfbr3-HA and phospho (P-)/total SMAD2 for ENG -/- Tgfbr3-HA MCF10A cells treated with or without 1 µg/ml DOX for 24 hours and 3 ng/ml TGFβ1 for 30 minutes. The bootstrapped means for each decile are overlaid. For (A) and (B), differences between the observed (bars) and expected (gray) distributions were assessed by K-S test. ∼ 0 is <10 -300 .

Pslik Tgfbr3 Ha Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pslik+tgfbr3+ha+neo/pSLIK+Tgfbr3-HA+neo+(Plasmid+%2358508)/bio_rxiv__64898__2026__03__01__708909-215-11-17
Average 94 stars, based on 1 article reviews

pslik tgfbr3 ha neo - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "TGFβ signaling systems are prone to inhibition and ligand competition by coreceptor"

Article Title: TGFβ signaling systems are prone to inhibition and ligand competition by coreceptor

Journal: bioRxiv

doi: 10.64898/2026.03.01.708909

Figure Legend Snippet: Dependence of prevalent coreceptor trends on additional ligand characteristics and biochemical validation of engineered ENG -/- Tgfbr3-HA MCF10A cells. Related to . (A) Low-affinity ligands with high k rD for A (≡ R II ) favor the +,– trend and disfavor the – trend. (B) Ligands with higher affinity for B (≡ R I ) than for C (≡ coreceptor) favor the +,– trend. (C) Immunoblot of parental MCF10A-5E cells (+/+) and FACS sorted MCF10A-5E ENG -/- cells (–/–) for ENG with tubulin and p38 used as loading controls. (D) Immunoblot of MCF10A-5E ENG -/- stably transduced with pSLIK-Tgfbr3-HA, treated with or without 1 µg/ml doxycycline (DOX) for 24 hours, and probed for Tgfbr3 and HA tag with tubulin and ERK1/2 used as loading controls. (E) Representative biplots of Tgfbr3-HA and phospho (P-)/total SMAD2 for ENG -/- Tgfbr3-HA MCF10A cells treated with or without 1 µg/ml DOX for 24 hours and 3 ng/ml TGFβ1 for 30 minutes. The bootstrapped means for each decile are overlaid. For (A) and (B), differences between the observed (bars) and expected (gray) distributions were assessed by K-S test. ∼ 0 is <10 -300 .

Techniques Used: Biomarker Discovery, Western Blot, Stable Transfection, Transduction

Transfection:

Article Title: TGFβ signaling systems are prone to inhibition and ligand competition by coreceptor
Article Snippet: .. Lentivirus was prepared in HEK293T/17 cells (ATCC) by triple transfection of pSLIK-Tgfbr3-HA neo with psPAX2 + pMD.2G (Addgene) and transduced into ENG -/- MCF10A-5E cells as described previously. ..

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Journal: bioRxiv

Article Title: TGFβ signaling systems are prone to inhibition and ligand competition by coreceptor

doi: 10.64898/2026.03.01.708909

Figure Lengend Snippet: Dependence of prevalent coreceptor trends on additional ligand characteristics and biochemical validation of engineered ENG -/- Tgfbr3-HA MCF10A cells. Related to . (A) Low-affinity ligands with high k rD for A (≡ R II ) favor the +,– trend and disfavor the – trend. (B) Ligands with higher affinity for B (≡ R I ) than for C (≡ coreceptor) favor the +,– trend. (C) Immunoblot of parental MCF10A-5E cells (+/+) and FACS sorted MCF10A-5E ENG -/- cells (–/–) for ENG with tubulin and p38 used as loading controls. (D) Immunoblot of MCF10A-5E ENG -/- stably transduced with pSLIK-Tgfbr3-HA, treated with or without 1 µg/ml doxycycline (DOX) for 24 hours, and probed for Tgfbr3 and HA tag with tubulin and ERK1/2 used as loading controls. (E) Representative biplots of Tgfbr3-HA and phospho (P-)/total SMAD2 for ENG -/- Tgfbr3-HA MCF10A cells treated with or without 1 µg/ml DOX for 24 hours and 3 ng/ml TGFβ1 for 30 minutes. The bootstrapped means for each decile are overlaid. For (A) and (B), differences between the observed (bars) and expected (gray) distributions were assessed by K-S test. ∼ 0 is <10 -300 .

Article Snippet: Lentivirus was prepared in HEK293T/17 cells (ATCC) by triple transfection of pSLIK-Tgfbr3-HA neo with psPAX2 + pMD.2G (Addgene) and transduced into ENG -/- MCF10A-5E cells as described previously.

Techniques: Biomarker Discovery, Western Blot, Stable Transfection, Transduction

Journal: Developmental cell

Article Title: Tumor Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer

doi: 10.1016/j.devcel.2017.10.027

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cross) N/A Experimental Models: Organisms/Strains Mouse: SCID Beige (CB17.Cg-Prkdc scid Lyst bg-J /Crl) Charles River Strain #250, RRID: IMSR_CRL:250 Mouse: BALB/c (BALB/cAnNCrl) Charles River Strain #028, RRID: IMSR_CRL:28 Oligonucleotides Primers for quantitative PCR, see Table S6 This paper N/A Recombinant DNA TET-pLKO.1 puro shLuc This paper Addgene #83092 TET-pLKO.1 puro shGFP This paper Addgene #83085 TET-pLKO.1 puro shLacZ This paper Addgene #98632 TET-pLKO.1 puro shGDF11 #1 This paper Addgene #83083 TET-pLKO.1 puro shGDF11 #2 This paper Addgene #83084 TET-pLKO.1 puro shSMAD4 #1 This paper Addgene #83089 TET-pLKO.1 puro shSMAD4 #2 This paper Addgene #83091 TET-pLKO.1 puro shID2 #1 This paper Addgene #83086 TET-pLKO.1 puro shID2 #2 This paper Addgene #83087 TET-pLKO.1 puro shId2 #1 This paper Addgene #83088 TET-pLKO.1 puro shId2 #2 This paper Addgene #83090 pLX304 LacZ-2′V5 blast This paper Addgene #83099 pLX304 GDF11-V5 blast This paper Addgene #83098 pLX304 PCSK5-V5 blast This paper Addgene #83100 pLX304 PCSK5 (T288P)-V5 blast This paper Addgene #83101 pLX304 PCSK5 (A270T)-V5 blast This paper Addgene #83102 pLX304 PCSK5 (H516P)-V5 blast This paper Addgene #83103 pLX304 PCSK5 (R511C)-V5 blast This paper Addgene #83104 pLX304 Luciferase-V5 blast This paper Addgene #98580 pLX302 ID2-V5 puro This paper Addgene #83096 pLX302 GDF11-V5 puro This paper Addgene #83097 pLX302 PCSK5-V5 puro This paper Addgene #98389 pLX302 PCSK5 (T288P)-V5 puro This paper Addgene #98709 pLX302 LacZ-2′V5 blast This paper Addgene #98579 pEN_TT_miRc2 3xFLAG This paper Addgene #83274 pEN_TT_miRc2 3xFLAG LacZ This paper Addgene #83093 pEN_TT_miRc2 3xFLAG Luciferase This paper Addgene #98391 pEN_TT_miRc2 3XFLAG SMAD4 This paper Addgene #83094 pEN_TT_miRc2 3XFLAG ID2 This paper Addgene #98390 pSLIK_TT 3xFLAG LacZ neo This paper Addgene #83105 pSLIK_TT 3xFLAG LacZ hygro This paper Addgene #98395 pSLIK_TT 3xFLAG Luciferase neo This paper Addgene #98392 pSLIK_TT 3xFLAG SMAD4 neo This paper Addgene #83273 pSLIK_TT 3xFLAG ID2 neo This paper Addgene #98393 pSLIK_TT 3xFLAG ID2 hygro This paper Addgene #98394 pBabe TGFBR3-HA neo This paper Addgene #83095 pBabe Venus neo This paper Addgene #98396 pBabe mRFP1 neo This paper Addgene #98397 pLKO.1 shTGFBR3 puro Wang et al., 2014 .

Techniques: In Situ, Recombinant, Enzyme-linked Immunosorbent Assay, Mutagenesis, Amplification, Expressing, Real-time Polymerase Chain Reaction, Luciferase, Software